Journal: Antimicrobial Agents and Chemotherapy

Article Title: Vitamin C Potentiates the Killing of Mycobacterium tuberculosis by the First-Line Tuberculosis Drugs Isoniazid and Rifampin in Mice

doi: 10.1128/AAC.02165-17

Figure Lengend Snippet: Vitamin C potentiates the activity of INH and RIF in mice. CBA/J mice were infected via the aerosol route with 28 CFU (A and B) or 105 CFU (C and D) of M. tuberculosis H37Rv. Treatment started 2 weeks postinfection. One group was left untreated. The mice received INH-RIF in their drinking water ad libitum. Vitamin C was given by i.p. injection at a concentration of 3 g/kg, 5 days a week, for 4 weeks. At the indicated times, mice were euthanized, and lungs and spleens were homogenized to quantify bacterial burdens. The CFU counts in lungs (A and C) and spleen (B and D) are shown for each individual animal, and the geometric mean is indicated for each group. The limit of detection is indicated by a dotted line. No colonies were isolated in the spleens of M. tuberculosis-infected mice treated with INH-RIF or INH-RIF-vitamin C in both experiments and are therefore not shown on panels C and D to simplify the figure. When the difference between two groups is significant, the P value is indicated based on a two-tailed unpaired nonparametric Mann-Whitney test.

Article Snippet: CBA/J female mice (6 to 8 weeks old) were obtained from Envigo (Indianapolis, IN).

Techniques: Activity Assay, Infection, Injection, Concentration Assay, Isolation, Two Tailed Test, MANN-WHITNEY

Journal: bioRxiv

Article Title: Cytolethal Distending Toxin Enhances Escherichia coli Urinary Tract Infection

doi: 10.64898/2026.02.09.704878

Figure Lengend Snippet: CBA/J mice (n=10) were transuretherally inoculated with equal numbers of wild-type (WT) HM56 and Δ cdt mutant HM56. Urine, bladders, and kidneys were collected for CFU enumeration at 24 h post-inoculation. A. CFU of each strain recovered from individual mice. Red symbols indicate no CFU recovered after plating. B. CI of the Δ cdt mutant compared to wild-type. Statistical significance was assessed by a one-sample t-test, comparing the mean log CI (lines) to a hypothetical value of zero for neutral fitness (dotted line): Statistically significant if P-values < 0.05.

Article Snippet: Female 6-7 week old CBA/J mice (Jackson Laboratory) were transuretherally inoculated with ca .

Techniques: Mutagenesis, IF-P

Journal: bioRxiv

Article Title: Cytolethal Distending Toxin Enhances Escherichia coli Urinary Tract Infection

doi: 10.64898/2026.02.09.704878

Figure Lengend Snippet: A. Timeline of infection and bacterial enumeration in female CBA/J mice transurethrally inoculated with wild-type (WT) HM56 and Δ cdt mutant strains. B-D. Bacterial burdens from individual mice in urine (B), bladder (C), and kidneys (D) ( n = 5-15) with the median indicated by solid lines and the limit of detection (urine) indicated by the dotted line. The red symbols in panel D designate mice in which bacterial counts were derived from only one kidney; the remaining kidney was preserved for histology. Statistical significance was assessed using the Mann–Whitney U test. P-values are displayed in the graph, and values < 0.05 were considered statistically significant.

Article Snippet: Female 6-7 week old CBA/J mice (Jackson Laboratory) were transuretherally inoculated with ca .

Techniques: Infection, Mutagenesis, Derivative Assay, MANN-WHITNEY